Description

Expected Starting Salary: 22.79 - 27.77

SUBFUNCTION DEFINITION: The Grimes lab is seeking a research assistant who can conduct independent and directed scientific studies focused on understanding the biological mechanisms responsible for normal and malignant hematopoiesis. The applicant must have experience with sterile tissue culture (preferably with iPSC and/or primary cells) and at least four-color flow cytometry (spectral cytometry experience a plus).

We utilize transgenic mouse models, patient samples, and multi-omics approaches to determine the effect of infection and molecular oncology on hematopoiesis. Our research team's goal is to provide relevant functional insight into hematological diseases like myelodysplastic syndromes (MDS), severe congenital neutropenias (SCN), and acute myeloid leukemia (AML) that may inform new avenues for therapeutic intervention. The RA will assist with lab and mouse colony upkeep, complete experiments including flow cytometric assessment and iPSC derived organoid culturing and maintenance, and analyze and present generated data.

REPRESENTATIVE RESPONSIBILITIES

Perform experiments and record data
Follow established research protocols. Understand the scientific principles involved in the research. Apply those principles to troubleshoot and modify procedures and techniques when appropriate. Maintain accurate and detailed records of all laboratory work and experiments. Analyze data and perform statistical and graphical analysis of data. Design and execute daily laboratory experiments in coordination with senior laboratory personnel. Apply standard protocols to specific experiments with the capability of reproducing experimental results. Maintains an active role in laboratory's meetings, presentations, and publications. Contributes material for reports, publications, journal articles, and abstracts. Able to orally present data in group presentations. Assists in training new laboratory personnel in laboratory techniques and equipment. A strong background in molecular biology and experience with mouse husbandry, handling, and tissue harvesting is required. Experience with flow cytometry (>4 colors), maintenance of cell lines, molecular DNA and RNA techniques, immunoblotting, and iPSC cell culturing and maintenance is preferred.

Lab Administration
Troubleshoot and correct erroneous results or problems with laboratory equipment. Troubleshoot and/or make modifications as necessary to established procedures. Maintain inventory of laboratory supplies and equipment. Maintain in vitro cell lines, including iPSC derived cells. Operate standard laboratory equipment, including flow cytometers and cell sorters. Willing and able to assist in daily operations of the laboratory including answering the phone, maintaining lab supplies, organization, and cleanliness. Maintains optimal protocol and assay accuracy by conducting regular quality checks. Provides appropriate safety monitoring checks as assigned (radiation safety, biosafety, etc.). Preparation of and amending of standard operating procedures (SOPs).

Serve as a Resource
Demonstrate competence in biomedical research, skills and behaviors related to hematopoiesis related research. Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. Stay abreast of changes in key systems. Serve as a resource to the department in specific area(s).

Research and improve current methods
Utilize internal and external resources to reproduce, evaluate, research, and improve techniques, bring in new techniques or modify existing techniques. Evaluate innovative techniques

Customer Service
Maintain/manage strong working relationships with clients, colleagues, and other key stakeholders. Respond to requests quickly and effectively. Identify and understand customer needs and expectations and meets them. Communicate any relevant information to clients, colleagues, and other key stakeholders.

Qualifications

EDUCATION/EXPERIENCE

Required for RAII:

High school diploma or equivalent

2 years of work experience in a related job discipline. or equivalent combination of education and experience

May require an Associate degree, vocational training, apprenticeships or equivalent

Required for RAIII:

Bachelor's degree in a related field

1 year of work experience in a related job discipline OR Master's degree in a related field

Cincinnati Children's is proud to be an Equal Opportunity Employer that values and treasures Diversity, Equity, and Inclusion. We are committed to creating an environment of dignity and respect for all our employees, patients, and families (EEO/AA).

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About Cincinnati Children's Hospital Medical Center

At Cincinnati Children’s, we come to work with one goal: to make children’s health better. We believe in a team approach, both in caring for patients and their families, and in advancing science and discovery. We strive to do better, and find energy and inspiration in our shared purpose. If you want to be the best you can be, you can do it at Cincinnati Children’s.

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